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TCR alpha and beta CDR3 sequences of these clones were cloned from genomic DNA or cDNA using a polymerase chain reaction (PCR) method. The amino acid sequences of the CDR3 regions were analyzed for V gene segments and J gene segments. The usage of CDR3 V gene segments varied widely from donor to donor. We did not find any preferences for V gene segments in the CDR3 regions of the clones.

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